Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests
نویسندگان
چکیده
منابع مشابه
Isothermal strand displacement amplification (iSDA): a rapid and sensitive method of nucleic acid amplification for point-of-care diagnosis.
We present a method of rapid isothermal amplification of DNA without initial heat denaturation of the template, and methods and probes for (a) real-time fluorescence detection and (b) lateral flow detection of amplicons. Isothermal strand displacement amplification (iSDA) can achieve >10(9)-fold amplification of the target sequence in <20 minutes at 49 °C, which makes it one of the fastest exis...
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ژورنال
عنوان ژورنال: Biosensors and Bioelectronics
سال: 2021
ISSN: 0956-5663
DOI: 10.1016/j.bios.2021.113168